AgrobacTransV1, Main, Exploration, bibRecord, 000722

Comparison of several Nicotiana species as hosts for high-scale Agrobacterium-mediated transient expression.

Identifieur interne : 000722 ( Main/Exploration ); précédent : 000721; suivant : 000723

Comparison of several Nicotiana species as hosts for high-scale Agrobacterium-mediated transient expression.

Auteurs : Y V Sheludko [Ukraine] ; Y R Sindarovska ; I M Gerasymenko ; M A Bannikova ; N V Kuchuk

Source :

Biotechnology and bioengineering [ 0006-3592 ] ; 2007.

RBID : pubmed:16983697

Descripteurs français

English descriptors

KwdEn :
- Capsid Proteins (genetics), Gene Expression Regulation, Plant (genetics), Genes, Reporter (MeSH), Genetic Vectors (MeSH), Plant Leaves (genetics), Plant Viruses (genetics), Plants, Genetically Modified (genetics), Promoter Regions, Genetic (genetics), Rhizobium (genetics), Tobacco (genetics), Transgenes (MeSH).
MESH :
- chemical , genetics : Capsid Proteins.
- genetics : Gene Expression Regulation, Plant, Plant Leaves, Plant Viruses, Plants, Genetically Modified, Promoter Regions, Genetic, Rhizobium, Tobacco.
- Genes, Reporter, Genetic Vectors, Transgenes.

Abstract

Agrobacterium-mediated transient expression may be regarded as a promising method for inexpensive large-scale production of recombinant proteins. We optimized the protocol of transient expression in Nicotiana benthamiana and compared six Australian species of Nicotiana as hosts for transient expression. The transient expression of GFP under 35S CaMV promoter was observed in all species tested, although the GFP content in leaves of N. benthamiana, N. exigua, and N. excelsior was significantly higher (3.8, 3.7, and 2.0% TSP, respectively). Usage of viral-based expression system resulted in considerable increase of GFP accumulation in N. excelsior and N. benthamiana (63.5 and 16.2% TSP, respectively). We displayed that N. excelsior has the best characteristics in regard to biomass yield as well as GFP accumulation level for both types of the expression cassettes tested.

DOI: 10.1002/bit.21075
PubMed: 16983697

Affiliations:

Ukraine

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Le document en format XML

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<term>Genes, Reporter (MeSH)</term>
<term>Genetic Vectors (MeSH)</term>
<term>Plant Leaves (genetics)</term>
<term>Plant Viruses (genetics)</term>
<term>Plants, Genetically Modified (genetics)</term>
<term>Promoter Regions, Genetic (genetics)</term>
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<term>Tobacco (genetics)</term>
<term>Transgenes (MeSH)</term>
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<keywords scheme="KwdFr" xml:lang="fr"><term>Feuilles de plante (génétique)</term>
<term>Gènes rapporteurs (MeSH)</term>
<term>Protéines de capside (génétique)</term>
<term>Rhizobium (génétique)</term>
<term>Régions promotrices (génétique) (génétique)</term>
<term>Régulation de l'expression des gènes végétaux (génétique)</term>
<term>Tabac (génétique)</term>
<term>Transgènes (MeSH)</term>
<term>Vecteurs génétiques (MeSH)</term>
<term>Virus des plantes (génétique)</term>
<term>Végétaux génétiquement modifiés (génétique)</term>
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<term>Promoter Regions, Genetic</term>
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<front><div type="abstract" xml:lang="en">Agrobacterium-mediated transient expression may be regarded as a promising method for inexpensive large-scale production of recombinant proteins. We optimized the protocol of transient expression in Nicotiana benthamiana and compared six Australian species of Nicotiana as hosts for transient expression. The transient expression of GFP under 35S CaMV promoter was observed in all species tested, although the GFP content in leaves of N. benthamiana, N. exigua, and N. excelsior was significantly higher (3.8, 3.7, and 2.0% TSP, respectively). Usage of viral-based expression system resulted in considerable increase of GFP accumulation in N. excelsior and N. benthamiana (63.5 and 16.2% TSP, respectively). We displayed that N. excelsior has the best characteristics in regard to biomass yield as well as GFP accumulation level for both types of the expression cassettes tested.</div>
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<Abstract><AbstractText>Agrobacterium-mediated transient expression may be regarded as a promising method for inexpensive large-scale production of recombinant proteins. We optimized the protocol of transient expression in Nicotiana benthamiana and compared six Australian species of Nicotiana as hosts for transient expression. The transient expression of GFP under 35S CaMV promoter was observed in all species tested, although the GFP content in leaves of N. benthamiana, N. exigua, and N. excelsior was significantly higher (3.8, 3.7, and 2.0% TSP, respectively). Usage of viral-based expression system resulted in considerable increase of GFP accumulation in N. excelsior and N. benthamiana (63.5 and 16.2% TSP, respectively). We displayed that N. excelsior has the best characteristics in regard to biomass yield as well as GFP accumulation level for both types of the expression cassettes tested.</AbstractText>
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	Serveur d'exploration sur l'agrobacterium et la transgénèse
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Serveur d'exploration sur l'agrobacterium et la transgénèse

Comparison of several Nicotiana species as hosts for high-scale Agrobacterium-mediated transient expression.

Comparison of several Nicotiana species as hosts for high-scale Agrobacterium-mediated transient expression.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki